| Plasmid
# |
Strain |
Plamid Name |
Description |
Box # |
Position |
Date |
Marker(s) |
Source |
|
| p1 |
DH5a |
pGEX-4T-3 |
expression of GST fusions in E. coli |
1 |
1,1 |
2000-08-22 |
Amp |
G. Cote |
|
| p2 |
DH5a |
pET16b |
expression of Nterm His fusions in E. coli |
1 |
1,2 |
2000-08-22 |
Amp |
D. Hyndman |
|
| p3 |
DH5a |
pET21b |
expression of Cterm His fusions in E. coli |
1 |
1,3 |
2000-08-22 |
Amp |
G. Cote |
|
| p4 |
MC1061 |
pET16b(No EcoRI) |
EcoRI site at 5709 destroyed by digestion, blunt-ending,
and re-ligation |
1 |
1,4 |
2000-08-31 |
Amp |
Chris |
|
| p5 |
TOP10F' |
pCR2.1-yidD |
TA clone after PCR with yidD_f and yidD_r |
1 |
1,5 |
2000-09-08 |
Amp, Kan |
Chris |
|
| p6 |
TOP10F' |
pCR2.1-yohL |
TA clone after PCR with yohL_f and yohL_r |
1 |
1,6 |
2000-09-08 |
Amp, Kan |
Chris |
|
| p7 |
TOP10F' |
pCR2.1-yefM |
TA clone after PCR with yefM_f and yefM_r |
1 |
1,7 |
2000-09-08 |
Amp, Kan |
Chris |
|
| p8 |
TOP10F' |
pCR2.1-relE |
TA clone after PCR with relE_f and relE_r |
1 |
1,8 |
2000-09-08 |
Amp, Kan |
Chris |
|
| p9 |
TOP10F' |
pCR2.1-yhhG |
TA clone after PCR with yhhG_f and yhhG_r |
1 |
1,9 |
2003-01-01 |
Amp, Kan |
Chris |
|
| p10 |
TOP10F' |
pCR2.1-yhfA |
TA clone after PCR with yhfA_f and yhfA_r |
1 |
2,1 |
1931-01-01 |
Amp, Kan |
Chris |
|
| p11 |
TOP10F' |
pCR2.1-yjbQ |
TA clone after PCR with yjbQ_f and yjbQ_r |
1 |
2,2 |
2000-09-12 |
Amp, Kan |
Chris |
|
| p12 |
TOP10F' |
pCR2.1-yjeB |
TA clone after PCR with yjeB_f and yjeB_r |
1 |
2,3 |
2000-09-12 |
Amp, Kan |
Chris |
|
| p13 |
MC1061 |
pET16b with pGEX-4T-3
MCS |
p4 [NdeI_BamHI] ligated to linker 1 |
1 |
2,4 |
2000-09-13 |
Amp |
Chris |
|
| p14 |
TOP10F' |
pCR2.1-yhbC |
TA clone after PCR with yhbC_f and yhbC_r |
1 |
2,5 |
2010-08-01 |
Amp, Kan |
Chris |
|
| p15 |
TOP10F' |
pCR2.1-ynfA |
TA-clone after PCR with ec1787865_f/_r |
1 |
2,6 |
2012-02-01 |
Amp, Kan |
Mei Yee Choi |
|
| p16 |
TOP10F' |
pCR2.1-ybeA |
TA-clone after PCR with ybeA_f and ybeA_r |
1 |
2,7 |
2016-08-01 |
Amp, Kan |
Chris |
|
| p17 |
TOP10F' |
pCR2.1-ytfP |
TA-clone after PCR with ytfP_f and ytfP_r |
1 |
2,8 |
2000-09-15 |
Amp, Kan |
Chris |
|
| p18 |
TOP10F' |
pCR2.1-yneC |
TA-clone after PCR with yneC_f and yneC_r |
1 |
2,9 |
2000-09-19 |
Amp, Kan |
Chris |
|
| p19 |
TOP10F' |
pCR2.1-yggU |
TA-clone after PCR with yggU_f and yggU_r |
1 |
3,1 |
2012-02-01 |
Amp, Kan |
Mei Yee Choi |
|
| p20 |
TOP10F' |
pCR2.1-ygiN |
TA-clone after PCR with ygiN_f and ygiN_r |
1 |
3,2 |
2011-07-01 |
Amp, Kan |
Chris |
|
| p21 |
TOP10F' |
pCR2.1-yljA |
TA-clone after PCR with yljA_f and yljA_r |
1 |
3,3 |
2000-09-19 |
Amp, Kan |
Chris |
|
| p22 |
MC1061 |
pET21b with pGEX-4T-3
MCS |
pET21b [NdeI_XhoI] ligated to linker 2 |
1 |
3,4 |
2000-09-22 |
Amp |
Chris |
|
| p23 |
MC1061 |
pGST-yidD |
yidD from p5 cloned BamHI/NotI into p1 |
1 |
3,5 |
2000-09-28 |
Amp |
Chris |
|
| p24 |
MC1061 |
pHis10-yidD |
yidD from p5 cloned BamHI/NotI into p13 |
1 |
3,6 |
2000-09-28 |
Amp |
Chris |
|
| p25 |
MC1061 |
pyidD-His6 |
yidD from p5 cloned BamHI/NotI into p22 |
1 |
3,7 |
2000-09-28 |
Amp |
Chris |
|
| p26 |
MC1061 |
pGST-yohL |
yohL from p6 cloned BamHI/NotI into p1 |
1 |
3,8 |
2000-11-09 |
Amp |
Chris |
|
| p27 |
MC1061 |
pHis10-yohL |
yohL from p6 cloned BamHI/NotI into p13 |
1 |
3,9 |
2000-11-09 |
Amp |
Chris |
|
| p28 |
MC1061 |
pyohL-His6 |
yohL from p6 cloned BamHI/NotI into p22 |
1 |
4,1 |
2000-11-09 |
Amp |
Chris |
|
| p29 |
MC1061 |
pGST-yefM |
yefM from p7 cloned BamHI/NotI into p1 |
1 |
4,2 |
2000-10-05 |
Amp |
Chris |
|
| p30 |
MC1061 |
pyefM-His6 |
yefM from p7 cloned BamHI/NotI into p22 |
1 |
4,3 |
2000-10-05 |
Amp |
Chris |
|
| p31 |
MC1061 |
pHis10-yefM |
yefM from p7 cloned BamHI/NotI into p13 |
1 |
4,4 |
2000-10-11 |
Amp |
Chris |
|
| p32 |
MC1061 |
pHis10-relE |
relE from p8 cloned BamHI/NotI into p13 |
1 |
4,5 |
2000-10-11 |
Amp |
Chris |
|
| p33 |
MC1061 |
prelE-His6 |
relE from p8 cloned BamHI/NotI into p22 |
1 |
4,6 |
2000-10-11 |
Amp |
Chris |
|
| p34 |
MC1061 |
pGST-relE |
relE from p8 cloned BamHI/NotI into p1 |
1 |
4,7 |
2000-10-20 |
Amp |
Chris |
|
| p35 |
MC1061 |
pGST-yhhG |
yhhG from p9 cloned BamHI/NotI into p1 |
1 |
4,8 |
2011-01-01 |
Amp |
Chris |
|
| p36 |
MC1061 |
pHis10-yhhG |
yhhG from p9 cloned BamHI/NotI into p13 |
1 |
4,9 |
2028-01-01 |
Amp |
Chris |
|
| p37 |
MC1061 |
pyhhG-His6 |
yhhG from p9 cloned BamHI/NotI into p22 |
1 |
5,1 |
2028-01-01 |
Amp |
Chris |
|
| p38 |
MC1061 |
pGST-yhfA |
yhfA from p10 cloned BamHI/NotI into p1 |
1 |
5,2 |
2008-02-01 |
Amp |
Chris |
|
| p39 |
MC1061 |
pHis10-yhfA |
yhfA from p10 cloned BamHI/NotI into p13 |
1 |
5,3 |
2008-02-01 |
Amp |
Chris |
|
| p40 |
MC1061 |
pyhfA-His6 |
yhfA from p10 cloned BamHI/NotI into p22 |
1 |
5,4 |
2008-02-01 |
Amp |
Chris |
|
| p41 |
MC1061 |
pGST-yjbQ |
yjbQ from p11 cloned BamHI/NotI into p1 |
1 |
5,5 |
2000-10-30 |
Amp |
Chris |
|
| p42 |
MC1061 |
pHis10-yjbQ |
yjbQ from p11 cloned BamHI/NotI into p13 |
1 |
5,6 |
2000-10-30 |
Amp |
Chris |
|
| p43 |
MC1061 |
pyjbQ-His6 |
yjbQ from p11 cloned BamHI/NotI into p22 |
1 |
5,7 |
2000-10-30 |
Amp |
Chris |
|
| p44 |
MC1061 |
pCR2.1-DSCR1L2 |
TA clone after PCR with DSCR1L2_f and DSCR1L2_r |
1 |
5,8 |
2000-11-08 |
Amp, Kan |
Chris |
|
| p45 |
MC1061 |
pGST-DSCR1L2 |
DSCR1L2 from p44 cloned BamHI/NotI into p1 |
1 |
5,9 |
2000-11-08 |
Amp |
Chris |
|
| p46 |
MC1061 |
pHis10-DSCR1L2 |
DSCR1L2 from p44 cloned BamHI/NotI into p13 |
1 |
6,1 |
2000-11-08 |
Amp |
Chris |
|
| p47 |
MC1061 |
pDSCR1L2-His6 |
DSCR1L2 from p44 cloned BamHI/NotI into p22 |
1 |
6,2 |
2000-11-08 |
Amp |
Chris |
|
| p48 |
TG2 |
pBS_SK+_CAF1p60 |
pPK7 from B. Stillman |
1 |
6,3 |
2000-11-08 |
Amp |
B. Stillman |
|
| p49 |
TG2 |
pBS_SK+_CAF1p150 |
pPK8 from B. Stillman |
1 |
6,4 |
2000-11-08 |
Amp |
B. Stillman |
|
| p50 |
TOP10F' |
pCR2.1-GAS2 |
TA clone after PCR with GAS2_f and GAS2_r |
1 |
6,5 |
2019-07-01 |
Amp, Kan |
Chris |
|
| p51 |
MC1061 |
pGST-yjeB |
yjeB from p12 cloned BamHI/NotI into p1 |
1 |
6,6 |
2000-11-20 |
Amp |
Chris |
|
| p52 |
MC1061 |
pGST-ygiN |
ygiN from p20 cloned BamHI/NotI into p1 |
1 |
6,7 |
2011-07-01 |
Amp |
Chris |
|
| p53 |
MC1061 |
pHis10-ygiN |
ygiN from p20 cloned BamHI/NotI into p13 |
1 |
6,8 |
2011-07-01 |
Amp |
Chris |
|
| p54 |
MC1061 |
pygiN-His6 |
ygiN from p20 cloned BamHI/NotI into p22 |
1 |
6,9 |
2011-07-01 |
Amp |
Chris |
|
| p55 |
MC1061 |
pHis10-yjeB |
yjeB from p12 cloned BamHI/NotI into p13 |
1 |
7,1 |
2000-12-15 |
Amp |
Chris |
|
| p56 |
MC1061 |
pGST-ytfP |
ytfP from p17 cloned BamHI/NotI into p1 |
1 |
7,2 |
2012-01-01 |
Amp |
Chris |
|
| p57 |
MC1061 |
pHis10-ytfP |
ytfP from p17 cloned BamHI/NotI into p13 |
1 |
7,3 |
2012-01-01 |
Amp |
Chris |
|
| p58 |
MC1061 |
pytfP-His6 |
ytfP from p17 cloned BamHI/NotI into p22 |
1 |
7,4 |
2012-01-01 |
Amp |
Chris |
|
| p59 |
MC1061 |
pGST-yneC |
yneC from p18 cloned BamHI/NotI into p1 |
1 |
7,5 |
2018-01-01 |
Amp |
Chris |
|
| p60 |
MC1061 |
pHis10-yneC |
yneC from p18 cloned BamHI/NotI into p13 |
1 |
7,6 |
2018-01-01 |
Amp |
Chris |
|
| p61 |
MC1061 |
pyneC-His6 |
yneC from p18 cloned BamHI/NotI into p22 |
1 |
7,7 |
2018-01-01 |
Amp |
Chris |
|
| p62 |
MC1061 |
pGST-yljA |
yljA from p21 cloned BamHI/NotI into p1 |
1 |
7,8 |
2019-01-01 |
Amp |
Chris |
|
| p63 |
MC1061 |
pHis10-yljA |
yljA from p21 cloned BamHI/NotI into p13 |
1 |
7,9 |
2019-01-01 |
Amp |
Chris |
|
| p64 |
MC1061 |
pyljA-His6 |
yljA from p21 cloned BamHI/NotI into p22 |
1 |
8,1 |
2019-01-01 |
Amp |
Chris |
|
| p65 |
MC1061 |
pGST-ynfA |
ec1787865 from p15 cloned BamHI/NotI into p1 |
1 |
8,2 |
2001-03-01 |
Amp |
Mei Yee Choi |
|
| p66 |
MC1061 |
pynfA-His6 |
ec1787865 from p15 cloned BamHI/NotI into p22 |
1 |
8,3 |
2001-03-01 |
Amp |
Mei Yee Choi |
|
| p67 |
TOP10F' |
pCR2.1-ybbJ |
TA-clone after PCR with ybbJ_f and ybbJ_r |
1 |
8,4 |
2002-02-01 |
Amp, Kan |
Chris |
|
| p68 |
TOP10F' |
pCR2.1-yabB |
TA-clone after PCR with yabB_f and yabB_r |
1 |
8,5 |
2002-02-01 |
Amp, Kan |
Chris |
|
| p69 |
MC1061 |
pyjeB-His6 |
yjeB from p12 cloned BamHI/NotI into p22 |
1 |
8,6 |
2008-02-01 |
Amp |
Chris |
|
| p70 |
MC1061 |
pGST-ybbJ |
ybbJ from p67 cloned BamHI/NotI into p1 |
1 |
8,7 |
2023-02-01 |
Amp |
Chris |
|
| p71 |
MC1061 |
pHis10-ybbJ |
ybbJ from p67 cloned BamHI/NotI into p13 |
1 |
8,8 |
2023-02-01 |
Amp |
Chris |
|
| p72 |
MC1061 |
pybbJ-His6 |
ybbJ from p67 cloned BamHI/NotI into p22 |
1 |
8,9 |
2023-02-01 |
Amp |
Chris |
|
| p73 |
MC1061 |
pGST-yabB |
yabB from p68 cloned BamHI/NotI into p1 |
1 |
9,1 |
2027-02-01 |
Amp |
Chris |
|
| p74 |
MC1061 |
pHis10-yabB |
yabB from p68 cloned BamHI/NotI into p13 |
1 |
9,2 |
2027-02-01 |
Amp |
Chris |
|
| p75 |
MC1061 |
pyabB-His6 |
yabB from p68 cloned BamHI/NotI into p22 |
1 |
9,3 |
2027-02-01 |
Amp |
Chris |
|
| p76 |
TOP10F' |
pCR2.1-ybaD |
TA-clone after PCR with ybaD_f and ybaD_r |
1 |
9,4 |
2002-03-01 |
Amp, Kan |
Chris |
|
| p77 |
MC1061 |
pGST-yggU |
yggU from p19 cloned BamHI/NotI into p1 |
1 |
9,5 |
2023-03-01 |
Amp |
Mei Yee Choi |
|
| p78 |
MC1061 |
pHis10-yggU |
yggU from p19 cloned BamHI/NotI into p13 |
1 |
9,6 |
2023-03-01 |
Amp |
Mei Yee Choi |
|
| p79 |
MC1061 |
pyggU-His6 |
yggU from p19 cloned BamHI/NotI into p22 |
1 |
9,7 |
2023-03-01 |
Amp |
Mei Yee Choi |
|
| p80 |
MC1061 |
pGST-ybaD |
ybaD from p76 cloned BamHI/NotI into p1 |
1 |
9,8 |
2023-03-01 |
Amp |
Chris |
|
| p81 |
MC1061 |
pHis10-ybaD |
ybaD from p76 cloned BamHI/NotI into p13 |
1 |
9,9 |
2023-03-01 |
Amp |
Chris |
|
| p82 |
MC1061 |
pybaD-His6 |
ybaD from p76 cloned BamHI/NotI into p22 |
2 |
1,1 |
2023-03-01 |
Amp |
Chris |
|
| p83 |
MC1061 |
pFastBacHTa |
Gibco/Life Technologies |
2 |
1,2 |
2025-04-01 |
Amp, Gen |
Chris |
|
| p84 |
MC1061 |
pFastBacHTb |
Gibco/Life Technologies |
2 |
1,3 |
2025-04-01 |
Amp, Gen |
Chris |
|
| p85 |
MC1061 |
pFastBacHTc |
Gibco/Life Technologies |
2 |
1,4 |
2025-04-01 |
Amp, Gen |
Chris |
|
| p86 |
MC1061 |
pFastBacHTb-DSCR1L2 |
DSCR1L2 from p45 cloned BamHI/NotI into p84 |
2 |
1,5 |
2026-04-01 |
Amp, Gen |
Chris |
|
| p87 |
MC1061 |
pcDNA3.1::PKY+Strep
tag |
PKY + tag cloned EcoRI into pcDNA3.1; this construct has
500bp of GC-rich 5' UTR preceding the start codon |
2 |
1,6 |
2018-05-01 |
Amp |
K.Sampson |
|
| p88 |
MC1061 |
pGST-GAS2 |
GAS2 from p50 cloned BamHI/NotI into p1 |
2 |
1,7 |
2019-07-01 |
Amp |
Chris |
|
| p89 |
MC1061 |
pHis10-GAS2 |
GAS2 from p50 cloned BamHI/NotI into p13 |
2 |
1,8 |
2019-07-01 |
Amp |
Chris |
|
| p90 |
MC1061 |
pGAS2-His6 |
GAS2 from p50 cloned BamHI/NotI into p22 |
2 |
1,9 |
2019-07-01 |
Amp |
Chris |
|
| p91 |
MC1061 |
pFastBacHTb-GAS2 |
GAS2 from p50 cloned BamHI/NotI into p84 |
2 |
2,1 |
2026-07-01 |
Amp, Gen |
Chris |
|
| p92 |
MC1061 |
pGST-yhbC |
yhbC from p14 cloned BamHI/NotI into p1 |
2 |
2,2 |
2010-08-01 |
Amp |
Chris |
|
| p93 |
MC1061 |
pHis10-yhbC |
yhbC from p14 cloned BamHI/NotI into p13 |
2 |
2,3 |
2010-08-01 |
Amp |
Chris |
|
| p94 |
MC1061 |
pyhbC-His6 |
yhbC from p14 cloned BamHI/NotI into p22 |
2 |
2,4 |
2010-08-01 |
Amp |
Chris |
|
| p95 |
MC1061 |
pGST-ybeA |
ybeA from p16 cloned BamHI/XhoI into p1 |
2 |
2,5 |
2016-08-01 |
Amp |
Chris |
|
| p96 |
MC1061 |
pHis10-ybeA |
ybeA from p16 cloned BamHI/XhoI into p13 |
2 |
2,6 |
2016-08-01 |
Amp |
Chris |
|
| p97 |
MC1061 |
pybeA-His6 |
ybeA from p16 cloned BamHI/XhoI into p22 |
2 |
2,7 |
2016-08-01 |
Amp |
Chris |
|
| p98 |
TOP10F' |
pCR2.1-ygaU |
TA clone after PCR with ygaU_f and ygaU_r |
2 |
2,8 |
1930-08-01 |
Amp, Kan |
Chris |
|
| p99 |
MC1061 |
pGST-ygaU |
ygaU from p98 cloned BamHI/NotI into p1 |
2 |
2,9 |
1930-08-01 |
Amp |
Chris |
|
| p100 |
MC1061 |
pHis10-ygaU |
ygaU from p98 cloned BamHI/NotI into p13 |
2 |
3,1 |
1930-08-01 |
Amp |
Chris |
|
| p101 |
MC1061 |
pygaU-His6 |
ygaU from p98 cloned BamHI/NotI into p22 |
2 |
3,2 |
1930-08-01 |
Amp |
Chris |
|
| p102 |
TOP10F' |
pCR2.1-ec1787199 |
TA clone after PCR with ec1787199_f/_r |
2 |
3,3 |
2001-09-01 |
Amp, Kan |
Chris |
|
| p103 |
MC1061 |
pGST-ec1787199 |
ec1787199 from p102 cloned BamHI/NotI into p1 |
2 |
3,4 |
2001-09-01 |
Amp |
Chris |
|
| p104 |
MC1061 |
pHis10-ec1787199 |
ec1787199 from p102 cloned BamHI/NotI into p13 |
2 |
3,5 |
2001-09-01 |
Amp |
Chris |
|
| p105 |
MC1061 |
pec1787199-His6 |
ec1787199 from p102 cloned BamHI/NotI into p22 |
2 |
3,6 |
2001-09-01 |
Amp |
Chris |
|
| p106 |
TOP10F' |
pCR2.1-yajQ |
TA clone after PCR with yajQ_f and yajQ_r |
2 |
3,7 |
2024-09-01 |
Amp, Kan |
Chris |
|
| p107 |
MC1061 |
pGST-yajQ |
yajQ from p106 cloned BamHI/NotI into p1 |
2 |
3,8 |
2024-09-01 |
Amp |
Chris |
|
| p108 |
MC1061 |
pHis10-yajQ |
yajQ from p106 cloned BamHI/NotI into p13 |
2 |
3,9 |
2024-09-01 |
Amp |
Chris |
|
| p109 |
MC1061 |
pyajQ-His6 |
yajQ from p106 cloned BamHI/NotI into p22 |
2 |
4,1 |
2024-09-01 |
Amp |
Chris |
|
| p110 |
TOP10F' |
pCR2.1-yjjX |
TA clone after PCR with yjjX_f and yjjX_r |
2 |
4,2 |
2026-09-01 |
Amp, Kan |
Chris |
|
| p111 |
MC1061 |
pGST-yjjX |
yjjX from p110 cloned EcoRI/NotI into p1 |
2 |
4,3 |
2026-09-01 |
Amp |
Chris |
|
| p112 |
MC1061 |
pHis10-yjjX |
yjjX from p110 cloned EcoRI/NotI into p13 |
2 |
4,4 |
2026-09-01 |
Amp |
Chris |
|
| p113 |
MC1061 |
pyjjX-His6 |
yjjX from p110 cloned EcoRI/NotI into p22 |
2 |
4,5 |
2026-09-01 |
Amp |
Chris |
|
| p114 |
TOP10F' |
pCR2.1-yhbN |
TA clone after PCR with yhbN_f and yhbN_r |
2 |
4,6 |
2024-10-01 |
Amp, Kan |
Chris |
|
| p115 |
MC1061 |
pGST-yhbN |
yhbN from p114 cloned BamHI/NotI into p1 |
2 |
4,7 |
2024-10-01 |
Amp |
Chris |
|
| p116 |
MC1061 |
pHis10-yhbN |
yhbN from p114 cloned BamHI/NotI into p13 |
2 |
4,8 |
2024-10-01 |
Amp |
Chris |
|
| p117 |
MC1061 |
pyhbN-His6 |
yhbN from p114 cloned BamHI/NotI into p22 |
2 |
4,9 |
2024-10-01 |
Amp |
Chris |
|
| p118 |
TOP10F' |
pCR2.1-yaiI |
TA clone after PCR with yaiI_f and yaiI_r |
2 |
5,1 |
2025-10-01 |
Amp, Kan |
Chris |
|
| p119 |
MC1061 |
pGST-yaiI |
yaiI from p118 cloned EcoRI/NotI into p1 |
2 |
5,2 |
2025-10-01 |
Amp |
Chris |
|
| p120 |
MC1061 |
pHis10-yaiI |
yaiI from p118 cloned EcoRI/NotI into p13 |
2 |
5,3 |
2025-10-01 |
Amp |
Chris |
|
| p121 |
MC1061 |
pyaiI-His6 |
yaiI from p118 cloned EcoRI/NotI into p22 |
2 |
5,4 |
2025-10-01 |
Amp |
Chris |
|
| p122 |
TOP10F' |
pCR2.1-ynjA |
TA clone after PCR with ynjA_f and ynjA_r |
2 |
5,5 |
2001-11-01 |
Amp, Kan |
Chris |
|
| p123 |
MC1061 |
pGST-ynjA |
ynjA from p122 cloned EcoRI/NotI into p1 |
2 |
5,6 |
2001-11-01 |
Amp |
Chris |
|
| p124 |
MC1061 |
pHis10-ynjA |
ynjA from p122 cloned EcoRI/NotI into p13 |
2 |
5,7 |
2001-11-01 |
Amp |
Chris |
|
| p125 |
MC1061 |
pynjA-His6 |
ynjA from p122 cloned EcoRI/NotI into p22 |
2 |
5,8 |
2001-11-01 |
Amp |
Chris |
|
| p126 |
MC1061 |
pET21c+Capn10 |
(in-frame with T7 tag, but not His-tag) |
2 |
5,9 |
2008-11-01 |
Amp |
G. Bell |
|
| p127 |
TOP10F' |
pCR2.1-yqgE |
TA clone after PCR with yqgE_f and yqgE_r |
2 |
6,1 |
2023-11-01 |
Amp, Kan |
Chris |
|
| p128 |
MC1061 |
pGST-yqgE |
yqgE from p127 cloned EcoRI/NotI into p1 |
2 |
6,2 |
2023-11-01 |
Amp |
Chris |
|
| p129 |
MC1061 |
pHis10-yqgE |
yqgE from p127 cloned EcoRI/NotI into p13 |
2 |
6,3 |
2023-11-01 |
Amp |
Chris |
|
| p130 |
MC1061 |
pyqgE-His6 |
yqgE from p127 cloned EcoRI/NotI into p22 |
2 |
6,4 |
2023-11-01 |
Amp |
Chris |
|
| p131 |
TOP10F' |
pCR2.1-CAPN10 |
TA clone after PCR with CAPN10_f/_r; p126 template |
2 |
6,5 |
2006-12-01 |
Amp, Kan |
Chris |
|
| p132 |
MC1061 |
pFastBacHTc-CAPN10 |
CAPN10 from p131 cloned EcoRI/NotI into p85 |
2 |
6,6 |
2006-12-01 |
Amp, Gen |
Chris |
|
| p133 |
MC1061 |
pGST-CAPN10 |
CAPN10 from p131 cloned EcoRI/NotI into p1 |
2 |
6,7 |
2006-12-01 |
Amp |
Chris |
|
| p134 |
MC1061 |
pHis10-CAPN10 |
CAPN10 from p131 cloned EcoRI/NotI into p13 |
2 |
6,8 |
2006-12-01 |
Amp |
Chris |
|
| p135 |
MC1061 |
pCAPN10-His6 |
CAPN10 from p131 cloned EcoRI/NotI into p22 |
2 |
6,9 |
2006-12-01 |
Amp |
Chris |
|
| p136 |
TOP10F' |
pCR2.1-yhhW |
TA clone after PCR with yhhW_f and yhhW_r |
2 |
7,1 |
2012-12-01 |
Amp, Kan |
Chris |
|
| p137 |
MC1061 |
pGST-yhhW |
yhhW from p136 cloned EcoRI/NotI into p1 |
2 |
7,2 |
2012-12-01 |
Amp |
Chris |
|
| p138 |
MC1061 |
pHis10-yhhW |
yhhW from p136 cloned EcoRI/NotI into p13 |
2 |
7,3 |
2012-12-01 |
Amp |
Chris |
|
| p139 |
MC1061 |
pyhhW-His6 |
yhhW from p136 cloned EcoRI/NotI into p22 |
2 |
7,4 |
2012-12-01 |
Amp |
Chris |
|
| p140 |
MC1061 |
pCBD-His6 |
p22 with CBD tag cloned upstream of MCS |
2 |
7,5 |
2014-01-02 |
Amp |
Chris |
|
| p141 |
MC1061 |
pET34b(+) |
for expression of CBD fusions in E. coli |
2 |
7,6 |
2025-01-02 |
Kan |
Novagen |
|
| p142 |
MC1061 |
pFastBacHTb-MhckA(T5) |
T5 clone from Qilu cloned into p84 |
2 |
7,7 |
2001-02-02 |
Amp, Gen |
Chris |
|
| p143 |
TOP10F' |
pCR2.1-yiiM |
TA clone after PCR with yiiM_f and yiiM_r |
2 |
7,8 |
2028-02-02 |
Amp, Kan |
Chris |
|
| p144 |
MC1061 |
pGST-yiiM |
yiiM from p143 cloned BamHI/NotI into p1 |
2 |
7,9 |
2028-02-02 |
Amp |
Chris |
|
| p145 |
MC1061 |
pHis10-yiiM |
yiiM from p143 cloned BamHI/NotI into p13 |
2 |
8,1 |
2028-02-02 |
Amp |
Chris |
|
| p146 |
MC1061 |
pyiiM-His6 |
yiiM from p143 cloned BamHI/NotI into p22 |
2 |
8,2 |
2028-02-02 |
Amp |
Chris |
|
| p147 |
MC1061 |
pCBD::Gas2::His6 |
Gas2 from p50 cloned BamHI/NotI into p140 |
2 |
8,3 |
2028-02-02 |
Amp |
Chris |
|
| p148 |
TOP10F' |
pCR2.1-yaeB |
TA clone after PCR with yaeB_f and yaeB_r |
2 |
8,4 |
2022-03-02 |
Amp, Kan |
Chris |
|
| p149 |
MC1061 |
pGST-yaeB |
yaeB from p148 cloned EcoRI/NotI into p1 |
2 |
8,5 |
2022-03-02 |
Amp |
Chris |
|
| p150 |
MC1061 |
pHis10-yaeB |
yaeB from p148 cloned EcoRI/NotI into p13 |
2 |
8,6 |
2022-03-02 |
Amp |
Chris |
|
| p151 |
MC1061 |
pyaeB-His6 |
yaeB from p148 cloned EcoRI/NotI into p22 |
2 |
8,7 |
2022-03-02 |
Amp |
Chris |
|
| p152 |
TOP10F' |
pCR2.1-yfiH |
TA clone after PCR with yfiH_f and yfiH_r |
2 |
8,8 |
2025-03-02 |
Amp, Kan |
Chris |
|
| p153 |
MC1061 |
pGST-yfiH |
yfiH from p152 cloned BamHI/NotI into p1 |
2 |
8,9 |
2025-03-02 |
Amp |
Chris |
|
| p154 |
MC1061 |
pHis10-yfiH |
yfiH from p152 cloned BamHI/NotI into p13 |
2 |
9,1 |
2025-03-02 |
Amp |
Chris |
|
| p155 |
MC1061 |
pyfiH-His6 |
yfiH from p152 cloned BamHI/NotI into p22 |
2 |
9,2 |
2025-03-02 |
Amp |
Chris |
|
| p156 |
TOP10F' |
pCR2.1-yggJ |
TA clone after PCR with yggJ_f and yggJ_r |
2 |
9,3 |
2011-04-02 |
Amp, Kan |
Chris |
|
| p157 |
MC1061 |
pGST-yggJ |
yggJ from p156 cloned BamHI/NotI into p1 |
2 |
9,4 |
2011-04-02 |
Amp |
Chris |
|
| p158 |
MC1061 |
pHis10-yggJ |
yggJ from p156 cloned BamHI/NotI into p13 |
2 |
9,5 |
2011-04-02 |
Amp |
Chris |
|
| p159 |
MC1061 |
pyggJ-His6 |
yggJ from p156 cloned BamHI/NotI into p22 |
2 |
9,6 |
2011-04-02 |
Amp |
Chris |
|
| p160 |
TOP10F' |
pCR2.1-ycbC |
TA clone after PCR with ycbC_f and ycbC_r |
2 |
9,7 |
2015-04-02 |
Amp, Kan |
Chris |
|
| p161 |
MC1061 |
pGST-ycbC |
ycbC from p160 cloned BamHI/NotI into p1 |
2 |
9,8 |
2015-04-02 |
Amp |
Chris |
|
| p162 |
MC1061 |
pHis10-ycbC |
ycbC from p160 cloned BamHI/NotI into p13 |
2 |
9,9 |
2015-04-02 |
Amp |
Chris |
|
| p163 |
MC1061 |
pycbC-His6 |
ycbC from p160 cloned BamHI/NotI into p22 |
3 |
1,1 |
2015-04-02 |
Amp |
Chris |
|
| p164 |
TOP10F' |
pCR2.1-ybgF |
TA clone after PCR with ybgF_f and ybgF_r |
3 |
1,2 |
2026-04-02 |
Amp, Kan |
Chris |
|
| p165 |
MC1061 |
pGST-ybgF |
ybgF from p164 cloned BamHI/NotI into p1 |
3 |
1,3 |
2026-04-02 |
Amp |
Chris |
|
| p166 |
MC1061 |
pHis10-ybgF |
ybgF from p164 cloned BamHI/NotI into p13 |
3 |
1,4 |
2026-04-02 |
Amp |
Chris |
|
| p167 |
MC1061 |
pybgF-His6 |
ybgF from p164 cloned BamHI/NotI into p22 |
3 |
1,5 |
2026-04-02 |
Amp |
Chris |
|
| p168 |
TOP10F' |
pCR2.1-mazG |
TA clone after PCR with mazG_f and mazG_r |
3 |
1,6 |
2008-05-02 |
Amp, Kan |
Chris |
|
| p169 |
MC1061 |
pGST-mazG |
mazG from p168 cloned BamHI/NotI into p1 |
3 |
1,7 |
2008-05-02 |
Amp |
Chris |
|
| p170 |
MC1061 |
pHis10-mazG |
mazG from p168 cloned BamHI/NotI into p13 |
3 |
1,8 |
2008-05-02 |
Amp |
Chris |
|
| p171 |
MC1061 |
pmazG-His6 |
mazG from p168 cloned BamHI/NotI into p22 |
3 |
1,9 |
2008-05-02 |
Amp |
Chris |
|
| p172 |
MC1061 |
ORFexpress CAPN9 cDNA |
catalog #GC-S0368 (in pDONR201) |
3 |
2,1 |
1930-06-02 |
Kan |
Genecopoeia |
|
| p173 |
TOP10F' |
pCR2.1-CAPN9 |
TA clone after PCR with CAPN9_f and CAPN9_r |
3 |
2,2 |
2029-07-02 |
Amp, Kan |
Chris |
|
| p174 |
MC1061 |
pGST-CAPN9 |
CAPN9 from p173 cloned BamHI/NotI into p1 |
3 |
2,3 |
2029-07-02 |
Amp |
Chris |
|
| p175 |
MC1061 |
pHis10-CAPN9 |
CAPN9 from p173 cloned BamHI/NotI into p13 |
3 |
2,4 |
2029-07-02 |
Amp |
Chris |
|
| p176 |
MC1061 |
pCAPN9-His6 |
CAPN9 from p173 cloned BamHI/NotI into p22 |
3 |
2,5 |
2029-07-02 |
Amp |
Chris |
|
| p177 |
MC1061 |
ORFexpress CEM15 cDNA |
catalog #GC-W0560 (in pDONR201) |
3 |
2,6 |
2006-08-02 |
Kan |
Genecopoeia |
|
| p178 |
TOP10F' |
pCR2.1-wzc_kd |
TA clone after PCR with wzc_kd_f/wzc_kd_r |
3 |
2,7 |
2026-08-02 |
Amp, Kan |
Chris |
|
| p179 |
MC1061 |
pGST-wzc_kd |
wzc_kd from p178 cloned BamHI/NotI into p1 |
3 |
2,8 |
2026-08-02 |
Amp |
Chris |
|
| p180 |
MC1061 |
pHis10-wzc_kd |
wzc_kd from p178 cloned BamHI/NotI into p13 |
3 |
2,9 |
2026-08-02 |
Amp |
Chris |
|
| p181 |
TOP10F' |
pCR2.1-etk_kd |
TA clone after PCR with etk_kd_f/etk_kd_r |
3 |
3,1 |
2026-08-02 |
Amp, Kan |
Chris |
|
| p182 |
MC1061 |
pGST-etk_kd |
etk_kd from p181 cloned BamHI/NotI into p1 |
3 |
3,2 |
2026-08-02 |
Amp |
Chris |
|
| p183 |
MC1061 |
pHis10-etk_kd |
etk_kd from p181 cloned BamHI/NotI into p13 |
3 |
3,3 |
2026-08-02 |
Amp |
Chris |
|
| p184 |
TOP10F' |
pCR2.1-CEM15Nterm |
TA clone after PCR with CEM15_f/CEM15nt_r |
3 |
3,4 |
1930-08-02 |
Amp, Kan |
Chris |
|
| p185 |
MC1061 |
pGST-CEM15Nterm |
CEM15Nterm from p184 cloned EcoRI/NotI into p1 |
3 |
3,5 |
1930-08-02 |
Amp |
Chris |
|
| p186 |
MC1061 |
pHis10-CEM15Nterm |
CEM15Nterm from p184 cloned EcoRI/NotI in p13 |
3 |
3,6 |
1930-08-02 |
Amp |
Chris |
|
| p187 |
MC1061 |
pCEM15Nterm-His6 |
CEM15Nterm from p184 cloned EcoRI/NotI in p22 |
3 |
3,7 |
1930-08-02 |
Amp |
Chris |
|
| p188 |
TOP10F' |
pCR2.1-CEM15Cterm |
TA clone after PCR with CEM15ct_f/CEM15_r |
3 |
3,8 |
1930-09-02 |
Amp, Kan |
Chris |
|
| p189 |
MC1061 |
pGST-CEM15Cterm |
CEM15Cterm from p188 cloned EcoRI/NotI in p1 |
3 |
3,9 |
1930-09-02 |
Amp |
Chris |
|
| p190 |
MC1061 |
pHis10-CEM15Cterm |
CEM15Cterm from p188 cloned EcoRI/NotI in p13 |
3 |
4,1 |
1930-09-02 |
Amp |
Chris |
|
| p191 |
MC1061 |
pCEM15Cterm-His6 |
CEM15Cterm from p188 cloned EcoRI/NotI in p22 |
3 |
4,2 |
1930-09-02 |
Amp |
Chris |
|
| p192 |
TOP10F' |
pCR2.1-SopB |
TA clone after PCR with SopB_f and SopB_r |
3 |
4,3 |
2016-10-02 |
Amp, Kan |
Chris |
|
| p193 |
MC1061 |
pGST-SopB |
SopB from p192 cloned BamHI/NotI into p1 |
3 |
4,4 |
2016-10-02 |
Amp |
Chris |
|
| p194 |
MC1061 |
pHis10-SopB |
SopB from p192 cloned BamHI/NotI into p13 |
3 |
4,5 |
2016-10-02 |
Amp |
Chris |
|
| p195 |
MC1061 |
pSopB-His6 |
SopB from p192 cloned BamHI/NotI into p22 |
3 |
4,6 |
2016-10-02 |
Amp |
Chris |
|
| p196 |
MC1061 |
pPI123 |
His6-YAGQ_O157 in pET15b |
3 |
4,7 |
2025-10-02 |
Amp |
BRI |
|
| p197 |
MC1061 |
pPI124 |
His6-YBBZ_O157 in pET15b |
3 |
4,8 |
2025-10-02 |
Amp |
BRI |
|
| p198 |
MC1061 |
pPI125 |
His6-YBDK_O157 in pET15b |
3 |
4,9 |
2025-10-02 |
Amp |
BRI |
|
| p199 |
MC1061 |
pPI126 |
His6-YCAO_O157 in pET15b |
3 |
5,1 |
2025-10-02 |
Amp |
BRI |
|
| p200 |
MC1061 |
pPI127 |
His6-YDCF_O157 in pET15b |
3 |
5,2 |
2025-10-02 |
Amp |
BRI |
|
| p201 |
MC1061 |
pPI128 |
His6-YECE_O157 in pET15b |
3 |
5,3 |
2025-10-02 |
Amp |
BRI |
|
| p202 |
MC1061 |
pPI129 |
His6-YHAK_O157 in pET15b |
3 |
5,4 |
2025-10-02 |
Amp |
BRI |
|
| p203 |
MC1061 |
pPI130 |
His6-RTCB_O157 in pET15b |
3 |
5,5 |
2025-10-02 |
Amp |
BRI |
|
| p204 |
MC1061 |
pPI131 |
His6-URED_O157 in pET15b |
3 |
5,6 |
2025-10-02 |
Amp |
BRI |
|
| p205 |
MC1061 |
pPI132 |
His6-UREF_O157 in pET15b |
3 |
5,7 |
2025-10-02 |
Amp |
BRI |
|
| p206 |
MC1061 |
pPI135 |
His6-Z4090_O157 in pET15b |
3 |
5,8 |
2025-10-02 |
Amp |
BRI |
|
| p207 |
MC1061 |
pPI136 |
His6-Z5331_O157 in pET15b |
3 |
5,9 |
2025-10-02 |
Amp |
BRI |
|
| p208 |
MC1061 |
pPI137 |
His6-Z1200_O157 in pET15b |
3 |
6,1 |
2025-10-02 |
Amp |
BRI |
|
| p209 |
MC1061 |
pPI138 |
His6-Z2800_O157 in pET15b |
3 |
6,2 |
2025-10-02 |
Amp |
BRI |
|
| p210 |
MC1061 |
pPI139 |
His6-Z3393_O157 in pET15b |
3 |
6,3 |
2025-10-02 |
Amp |
BRI |
|
| p211 |
MC1061 |
pPI140 |
His6-Z5892_O157 in pET15b |
3 |
6,4 |
2025-10-02 |
Amp |
BRI |
|
| p212 |
MC1061 |
pPI141 |
His6-Z0258_O157 in pET15b |
3 |
6,5 |
2025-10-02 |
Amp |
BRI |
|
| p213 |
MC1061 |
pPI142 |
His6-Z0251_O157 in pET15b |
3 |
6,6 |
2025-10-02 |
Amp |
BRI |
|
| p214 |
MC1061 |
pPI143 |
His6-Z0252_O157 in pET15b |
3 |
6,7 |
2025-10-02 |
Amp |
BRI |
|
| p215 |
MC1061 |
pPI144 |
His6-Z0264_O157 in pET15b |
3 |
6,8 |
2025-10-02 |
Amp |
BRI |
|
| p216 |
MC1061 |
pPI145 |
His6-Z0261_O157 in pET15b |
3 |
6,9 |
2025-10-02 |
Amp |
BRI |
|
| p217 |
MC1061 |
pPI146 |
His6-Z0257_O157 in pET15b |
3 |
7,1 |
2025-10-02 |
Amp |
BRI |
|
| p218 |
MC1061 |
pPI148 |
His6-Z0509_O157 in pET15b |
3 |
7,2 |
2025-10-02 |
Amp |
BRI |
|
| p219 |
MC1061 |
pPI149 |
His6-Z0510_O157 in pET15b |
3 |
7,3 |
2025-10-02 |
Amp |
BRI |
|
| p220 |
MC1061 |
pPI150 |
His6-CHUS_O157 in pET15b |
3 |
7,4 |
2025-10-02 |
Amp |
BRI |
|
| p221 |
MC1061 |
pPI151 |
His6-CHUX_O157 in pET15b |
3 |
7,5 |
2025-10-02 |
Amp |
BRI |
|
| p222 |
MC1061 |
pPI152 |
His6-Z5688_O157 in pET15b |
3 |
7,6 |
2025-10-02 |
Amp |
BRI |
|
| p223 |
DH5a |
pT-Trx |
for co-expression of thioredoxin with pET system |
3 |
7,7 |
2025-10-02 |
Cm |
Lilly |
|
| p224 |
MC1061 |
pLacIQpar8-wfsAFP2 |
E. coli secretion vector for wfsAFP2 |
3 |
7,8 |
2029-10-02 |
Amp |
S. Lin |
|
| p225 |
MC1061 |
pLacIQpar8::MCS |
p224 with wfsAFP2 replaced with pGEX-4T-3 MCS |
3 |
7,9 |
2020-11-02 |
Amp |
Chris |
|
| p226 |
TOP10F' |
pCR2.1-SopBD28 |
TA clone after PCR with SopBD28_f and SopB_r |
3 |
8,1 |
2004-12-02 |
Amp, Kan |
Chris |
|
| p227 |
MC1061 |
pGST-SopBD28 |
SopBD28 from p226 cloned BamHI/NotI into p1 |
3 |
8,2 |
2004-12-02 |
Amp |
Chris |
|
| p228 |
MC1061 |
pHis10-SopBD28 |
SopBD28 from p226 cloned BamHI/NotI into p13 |
3 |
8,3 |
2004-12-02 |
Amp |
Chris |
|
| p229 |
MC1061 |
pSopBD28-His6 |
SopBD28 from p226 cloned BamHI/NotI into p22 |
3 |
8,4 |
2004-12-02 |
Amp |
Chris |
|
| p230 |
TOP10F' |
pCR2.1-SopBD166 |
TA clone after PCR with SopBD166_f and SopB_r |
3 |
8,5 |
2004-12-02 |
Amp, Kan |
Chris |
|
| p231 |
MC1061 |
pGST-SopBD166 |
SopBD166 from p230 cloned BamHI/NotI into p1 |
3 |
8,6 |
2004-12-02 |
Amp |
Chris |
|
| p232 |
MC1061 |
pHis10-SopBD166 |
SopBD166 from p230 cloned BamHI/NotI into p13 |
3 |
8,7 |
2004-12-02 |
Amp |
Chris |
|
| p233 |
MC1061 |
pSopBD166-His6 |
SopBD166 from p230 cloned BamHI/NotI into p22 |
3 |
8,8 |
2004-12-02 |
Amp |
Chris |
|
| p234 |
TOP10F' |
pCR2.1-CAPN10(21-511) |
TA clone after PCR with Cap10_f21/Cap10_r511 |
3 |
8,9 |
2023-12-02 |
Amp, Kan |
Chris |
|
| p235 |
MC1061 |
pGST-CAPN10(21-511) |
Insert from p234 cloned EcoRI/NotI into p1 |
3 |
9,1 |
2023-12-02 |
Amp |
Chris |
|
| p236 |
MC1061 |
pHis10-CAPN10(21-511) |
Insert from p234 cloned EcoRI/NotI into p13 |
3 |
9,2 |
2023-12-02 |
Amp |
Chris |
|
| p237 |
MC1061 |
pCAPN10(21-511)-His6 |
Insert from p234 cloned EcoRI/NotI into p22 |
3 |
9,3 |
2023-12-02 |
Amp |
Chris |
|
| p238 |
MC1061 |
pGST-yaiI NEW |
yaiI cloned with EcoRI/NotI into p1 |
3 |
9,4 |
2003-01-28 |
Amp |
Jim Blonde |
|
| p239 |
MC1061 |
pHis10-yaiI NEW |
yaiI cloned with EcoRI/NotI into p13 |
3 |
9,5 |
2003-01-28 |
Amp |
Jim Blonde |
|
| p240 |
MC1061 |
pyaiI-His6 NEW |
yaiI cloned with EcoRI/NotI into p22 |
3 |
9,6 |
2003-01-28 |
Amp |
Jim Blonde |
|
| p241 |
MC1061 |
pGST-yqgE NEW |
yqgE cloned with EcoRI/NotI into p1 |
3 |
9,7 |
2003-01-28 |
Amp |
Jim Blonde |
|
| p242 |
MC1061 |
pHis10-yqgE NEW |
yqgE cloned with EcoRI/NotI into p13 |
3 |
9,8 |
2003-01-28 |
Amp |
Jim Blonde |
|
| p243 |
MC1061 |
pyqgE-His6 NEW |
yqgE cloned with EcoRI/NotI into p22 |
3 |
9,9 |
2003-01-28 |
Amp |
Jim Blonde |
|
| p244 |
MC1061 |
pGST-yiiM NEW |
yiiM cloned with BamHI/NotI into p1 |
4 |
1,1 |
2003-01-28 |
Amp |
Jim Blonde |
|
| p245 |
MC1061 |
pHis10-yiiM NEW |
yiiM cloned with BamHI/NotI into p13 |
4 |
1,2 |
2003-01-28 |
Amp |
Jim Blonde |
|
| p246 |
MC1061 |
pyiiM-His6 NEW |
yiiM cloned with BamHII/NotI into p22 |
4 |
1,3 |
2003-01-28 |
Amp |
Jim Blonde |
|
| p247 |
MC1061 |
pGST-CAPN10D356 |
CAPN10D356, cloned with BamHI/NotI in p1 |
4 |
1,4 |
2003-02-05 |
Amp |
Jim Blonde |
|
| p248 |
MC1061 |
pHis10-CAPN10D356 |
CAPN10D356, cloned with BamHI/NotI in p13 |
4 |
1,5 |
2003-02-05 |
Amp |
Jim Blonde |
|
| p249 |
MC1061 |
pCAPN10D356-His6 |
CAPN10D356, cloned with BamHI/NotI in p22 |
4 |
1,6 |
2003-02-05 |
Amp |
Jim Blonde |
|
| p250 |
TOP10F' |
pCR2.1-CAPN9D3 |
TA-clone after PCR with CAPN9D3-F/CAPN9D3-R on p126 template |
4 |
1,7 |
2003-03-03 |
Amp, Kan |
Jim Blonde |
|
| p251 |
TOP10F' |
pCR2.1-CAPN10D3 |
TA-clone after PCR with CAPN10D3-F/CAPN10D3-R on p173 template |
4 |
1,8 |
2003-03-03 |
Amp, Kan |
Jim Blonde |
|
| p252 |
MC1061 |
pGST-CALPAIN9(350-495) |
Domain 3 of CALPAIN9 from p250 in p1 with BamHI/NotI |
4 |
1,9 |
2003-04-11 |
Amp |
Jim Blonde |
|
| p253 |
MC1061 |
pHis10-CALPAIN9(350-495) |
Domain 3 of CALPAIN9 from p250 in p13 with BamHI/NotI |
4 |
2,1 |
2003-04-11 |
Amp |
Jim Blonde |
|
| p254 |
MC1061 |
pCALPAIN9(350-495)-His6 |
Domain 3 of CALPAIN9 from p250 in p22 with BamHI/NotI |
4 |
2,2 |
2003-04-11 |
Amp |
Jim Blonde |
|
| p255 |
MC1061 |
pPIC6A |
Invitrogen pPIC6A Pichia expression vector |
4 |
2,3 |
2003-07-07 |
Blasticidin |
Invitrogen |
|
| p256 |
MC1061 |
pPIC6C |
Invitrogen pPIC6C Pichia expression vector |
4 |
2,4 |
2003-07-08 |
Blasticidin |
Invitrogen |
|
| p257 |
MC1061 |
pPIC6A(no BamHI) |
p255 with BamHI site removed (cut, Klenow, religated) |
4 |
2,5 |
2003-07-21 |
Blasticidin |
Jim Blonde |
|
| p258 |
MC1061 |
pPIC6B |
Invitrogen pPIC6B Pichia expression vector |
4 |
2,6 |
2003-07-21 |
Blasticidin |
Invitrogen |
|
| p259 |
MC1061 |
pGEXCHis |
pGEX4T3
(p1, Pharmacia) with linker 5 (adds C-terminal 6XHis tag) cloned in with Sal1/Not1-cut |
4 |
2,7 |
2003-07-21 |
Amp |
Jim Blonde |
|
| p260 |
MC1061 |
pPIC6AL4(incorrect) |
Sequencing indicates that this is a bad plasmid |
4 |
2,8 |
2003-07-21 |
Blasticidin |
Jim Blonde |
|
| p261 |
M15(pREP4) |
JW2515 (BRI) |
YFHP (Nhis6 tagged) cloned by Dr. Mori |
4 |
2,9 |
2003-08-25 |
Cm AND Kan |
Dr. Mori via BRI |
| p262 |
M15(pREP4) |
JW1083 (BRI) |
YCEG(Nhis6 tagged) cloned by Dr. Mori |
4 |
3,1 |
2003-08-25 |
Cm AND Kan |
Dr. Mori via BRI |
| p263 |
M15(pREP4) |
JW3172 (BRI) |
YHBJ (Nhis6 tagged) cloned by Dr. Mori |
4 |
3,2 |
2003-08-25 |
Cm AND Kan |
Dr. Mori via BRI |
| p264 |
M15(pREP4) |
JW2843 (BRI) |
YQEB (Nhis6 tagged) cloned by Dr. Mori |
4 |
3,3 |
2003-08-25 |
Cm AND Kan |
Dr. Mori via BRI |
| p265 |
M15(pREP4) |
JW2500 (BRI) |
YFGA (Nhis6 tagged) cloned by Dr. Mori |
4 |
3,4 |
2003-08-25 |
Cm AND Kan |
Dr. Mori via BRI |
| p266 |
M15(pREP4) |
JW0477 (BRI) |
YBBJ (Nhis6 tagged) cloned by Dr. Mori |
4 |
3,5 |
2003-08-25 |
Cm AND Kan |
Dr. Mori via BRI |
| p267 |
BL21(DE3) |
pZY9 (BRI) |
P76130(Nhis6) cloned by BRI |
4 |
3,6 |
2003-08-25 |
Amp |
BRI |
|
| p268 |
BL219(DE3) |
pZY11 (BRI) |
RELE(NHis6) cloned by BRI |
4 |
3,7 |
2003-08-25 |
Amp |
BRI |
|
| p269 |
MC1061 |
pPIC6AL4 |
p257 with Linker 4 (pGEX MCS) ligated between BstB1 and
SalI sites |
4 |
3,8 |
2003-09-16 |
Blasticidin |
Jim Blonde |
|
| p270 |
MC1061 |
pPICL4-Zeo |
p270 with blasticidin cassette replaced with zeocin
resistance cassette from p271 |
4 |
3,9 |
2003-11-18 |
Zeocin |
Jim Blonde |
|
| p271 |
MC1061 |
pPICZA |
Invitrogen pPICZA Pichia expression vector |
4 |
4,1 |
2003-11-18 |
Zeocin |
Invitrogen |
|
| p272 |
TOP10F' |
pCR2.1-MHCKA-T5 |
MHCKA-T5 TA-cloned from PCR of MHCKA-T5 clone (Dr. Cote)
(primers MHCKAT5-F, MHCKAT5-R) |
4 |
4,2 |
2003-12-17 |
Amp, Kan |
Jim Blonde |
|
| p273 |
TOP10F' |
pCR2.1-MHCKA-R3 |
MHCKA-R733A TA-cloned from PCR of MHCKA-R733A clone (Dr. Cote) (primers MHCKAT5-F, MHCKAT5-R) |
4 |
4,3 |
2003-12-17 |
Amp, Kan |
Jim Blonde |
|
| p274 |
MC1061 |
pPICL4zeo-MHCKAT5 |
MHCKA-T5 from p272 in p270 (BamHI/NotI) |
4 |
4,4 |
2004-03-03 |
Amp |
Jim Blonde |
|
| p275 |
MC1061 |
pPICL4zeo-MHCKAR3 |
MHCKA-R733A from p273 in p270 (BamHI/NotI) |
4 |
4,5 |
2004-03-03 |
Amp |
Jim Blonde |
|
| p276 |
TOP10F' |
pCR2.1-ETK(52-422) |
ETK (residues 52-422) TA-cloned from PCR of E.coli genome
(primers: ETK-N-F, ETK-N-R) |
4 |
4,6 |
2004-04-07 |
Amp, Kan |
Jim Blonde |
|
| p277 |
MC1061 |
pGST-ETK(52-422) |
ETK(52-422) from p276 in p1 (with BamHI, NotI) |
4 |
4,7 |
2004-04-07 |
Amp |
Jim Blonde |
|
| p278 |
MC1061 |
pHis10-ETK(52-422) |
ETK(52-422) from p276 in p13 (with BamHI, NotI) |
4 |
4,8 |
2004-04-07 |
Amp |
Jim Blonde |
|
| p279 |
MC1061 |
pETK-His6(52-422) |
ETK(52-422) from p276 in p22 (with BamHI, NotI) |
4 |
4,9 |
2004-04-07 |
Amp |
Jim Blonde |
|
| p280 |
MC1061 |
pPICL4zeo-ETK(52-422) |
ETK(52-422) from p276 in p270 (with BamHI, NotI) |
4 |
5,1 |
2004-04-07 |
Zeocin |
Jim Blonde |
|
|
|
|
|
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|
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