|
|
|
LeMASTER MEDIUM |
|
|
|
AUTOCLAVE SOLUTION A |
|
|
|
|
|
|
|
|
1L |
2L |
4L |
|
|
|
|
|
1 |
Alanine |
0.500 |
1 |
2.000 |
|
|
|
|
|
2 |
Arginine HCl |
0.580 |
1.16 |
2.320 |
|
|
|
|
|
3 |
Aspartic Acid |
0.400 |
0.8 |
1.600 |
|
|
|
|
|
4 |
L-Cystine |
0.033 |
0.066 |
0.132 |
|
|
|
|
|
5 |
Glutamic Acid
(Na) |
0.750 |
1.5 |
3.000 |
|
|
|
|
|
6 |
Glutamine |
0.333 |
0.666 |
1.332 |
|
|
|
|
|
7 |
Glycine |
0.540 |
1.08 |
2.160 |
|
|
|
|
|
8 |
Histidine |
0.060 |
0.12 |
0.240 |
|
|
|
|
|
9 |
Isoleucine |
0.230 |
0.46 |
0.920 |
|
|
|
|
|
10 |
Leucine |
0.230 |
0.46 |
0.920 |
|
|
|
|
|
11 |
Lysine HCl |
0.420 |
0.84 |
1.680 |
|
|
|
|
|
12 |
Phenylalanine |
0.133 |
0.266 |
0.532 |
|
|
|
|
|
13 |
Proline |
0.100 |
0.2 |
0.400 |
|
|
|
|
|
14 |
L-Serine |
2.083 |
4.166 |
8.332 |
|
|
|
|
|
15 |
Threonine |
0.230 |
0.46 |
0.920 |
|
|
|
|
|
16 |
Tyrosine |
0.170 |
0.34 |
0.680 |
|
|
|
|
|
17 |
Valine |
0.230 |
0.46 |
0.920 |
|
|
|
|
|
18 |
Adenine |
0.500 |
1 |
2.000 |
|
|
|
|
|
19 |
Guanosine |
0.670 |
1.34 |
2.680 |
|
|
|
|
|
20 |
Thymine |
0.170 |
0.34 |
0.680 |
|
|
|
|
|
21 |
Uracil |
0.500 |
1 |
2.000 |
|
|
|
|
|
22 |
Sodium Acetate |
1.500 |
3 |
6.000 |
|
|
|
|
|
23 |
Succinic Acid |
1.500 |
3 |
6.000 |
|
|
|
|
|
24 |
Ammonium Cl |
0.750 |
1.5 |
3.000 |
|
|
|
|
|
25 |
Sodium Hydroxide |
0.850 |
1.7 |
3.400 |
|
|
|
|
|
26 |
Dibasic K2HPO4 |
10.500 |
21 |
42.000 |
|
|
|
|
|
|
AUTOCLAVE: |
2L,
250mL, 125mL Flasks, 500mL centrifuge, 500mL threaded bottle |
|
|
(2) bubblers |
beaker for pouring into smaller flasks |
|
|
|
|
|
|
|
|
|
|
|
|
***LET COOL TO
37 DEGREES THEN ADD FILTERED 27-31 AND 32,33*** |
|
|
1L |
2L |
2.5L |
|
|
|
|
|
|
FILTERSTERILIZE SOLUTION B |
|
|
|
|
|
27 |
D-Glucose |
10.00 |
20 |
40.00 |
|
|
|
|
|
28 |
MgSO4-7H20 |
0.25 |
0.5 |
1.00 |
|
|
|
|
|
29 |
FeSO4 |
4.2 mg |
8.4 mg |
16.8 mg |
|
|
|
|
|
30 |
H2SO4 (liquid) |
8uL |
16uL |
32uL |
|
|
|
|
|
31 |
Thiamine |
0.005 |
0.01 |
0.020 |
|
|
|
|
|
|
|
41.3mL |
82.6mL |
103.25mL |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
32 |
Seleno Methionine |
50 mg |
|
200 mg |
|
|
|
|
|
33 |
Ampicillin |
100 mg |
|
400 mg |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|