LeMASTER MEDIUM
AUTOCLAVE SOLUTION A
1L 2L 4L
1 Alanine 0.500 1 2.000          
2 Arginine HCl 0.580 1.16 2.320          
3 Aspartic Acid 0.400 0.8 1.600          
4 L-Cystine 0.033 0.066 0.132          
5 Glutamic Acid (Na) 0.750 1.5 3.000          
6 Glutamine 0.333 0.666 1.332          
7 Glycine 0.540 1.08 2.160          
8 Histidine 0.060 0.12 0.240          
9 Isoleucine 0.230 0.46 0.920          
10 Leucine 0.230 0.46 0.920          
11 Lysine HCl 0.420 0.84 1.680          
12 Phenylalanine 0.133 0.266 0.532          
13 Proline 0.100 0.2 0.400          
14 L-Serine 2.083 4.166 8.332          
15 Threonine 0.230 0.46 0.920          
16 Tyrosine 0.170 0.34 0.680          
17 Valine 0.230 0.46 0.920          
18 Adenine 0.500 1 2.000          
19 Guanosine 0.670 1.34 2.680          
20 Thymine 0.170 0.34 0.680          
21 Uracil 0.500 1 2.000          
22 Sodium Acetate 1.500 3 6.000          
23 Succinic Acid 1.500 3 6.000          
24 Ammonium Cl 0.750 1.5 3.000          
25 Sodium Hydroxide 0.850 1.7 3.400          
26 Dibasic K2HPO4 10.500 21 42.000          
AUTOCLAVE:  2L, 250mL, 125mL Flasks, 500mL centrifuge, 500mL threaded bottle 
(2) bubblers beaker for pouring into smaller flasks
***LET COOL TO 37 DEGREES THEN ADD FILTERED 27-31 AND 32,33***
1L 2L 2.5L
FILTERSTERILIZE SOLUTION B
27 D-Glucose 10.00 20 40.00          
28 MgSO4-7H20 0.25 0.5 1.00          
29 FeSO4 4.2 mg 8.4 mg 16.8 mg          
30 H2SO4 (liquid) 8uL 16uL 32uL          
31 Thiamine 0.005 0.01 0.020          
41.3mL 82.6mL 103.25mL
32 Seleno Methionine 50 mg   200 mg          
33 Ampicillin 100 mg   400 mg