Low Salt LB (1L)
2004-03-12
- for selecting on blasticidin and zeocin
- makes 1 L
10 g Tryptone
5 g NaCl
5 g Yeast Extract
15 g agar (optional,
for making plates)
- Combine the above ingredients in 950 mL distilled,
deionized water
- Autoclave on liquid cycle for 20 minutes
- Allow media to cool to less than 550C
- Add blasticidin to a final concentration of 100
ug/mL. Blasticidin stocks are prepared to a concentration of 10 mg/mL in
ddH2O.
- Store plates/media at 40C in the dark. Plates
containing blasticidin are stables for up to 2 weeks.