Low Salt LB (1L)


- for selecting on blasticidin and zeocin

- makes 1 L


10 g    Tryptone

5 g       NaCl

5 g       Yeast Extract

15 g    agar (optional, for making plates)


  1. Combine the above ingredients in 950 mL distilled, deionized water
  2. Autoclave on liquid cycle for 20 minutes
  3. Allow media to cool to less than 550C
  4. Add blasticidin to a final concentration of 100 ug/mL.  Blasticidin stocks are prepared to a concentration of 10 mg/mL in ddH2O.
  5. Store plates/media at 40C in the dark.  Plates containing blasticidin are stables for up to 2 weeks.