Equipment and Facilities

10/31/08

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X-ray facilities

Our X-ray facilities consist of two systems: a Rigaku rotating-anode X-ray generator (MicroMax 007) equipped with a Mar-345 image plate and an Oxford cryogenic system; Bruker Proteum system with CCD detector. 

As usual, we regularly make trips to synchrotrons in the USA.

FPLC system

We have a modern Amersham Biosciences Akta FPLC (fast protein liquid chromatography) machine.  This sophisticated computer-controlled FPLC helps us quickly purify the large quantities of proteins we need to grow crystals.

Plate Reader

We have a UV/Vis absorbance plate reader, the Bio Tek PowerWave XS.  This machine makes Bradford assays fast and simple, and help us with enzyme kinetic studies.

Fermenter

We operate a New Brunswick biofermenter.  This machine is great for growing bacterial and yeast cultures, yielding an enormous amount of biomass.  We have recently upgraded this machine to make it computer-controlled and capable of growing 7 L fed-batch cultures.

Dynamic Light Scatterer

The Protein Solutions Dynapro light scatterer is used to determine to the size and oligomeric state of a protein in solution.  We use this information helps us to plan protein crystallization trials and to interpret x-ray diffraction results.

Computers

Our work is very computationally intensive.  We therefore have several powerful computers for diffraction analysis and protein visualization.  These machines include:

  • A network of several Linux workstations with multiple processors for numeric computation and graphic visulization

  • Several high-end PCs

 

 

Other stuff

We have a variety of other equipment to support and enhance our research.  These include:

  • Gradient thermocycler
  • UV/Vis spectrophotometer
  • Incubator/Shakers
  • Microcentrifuges
  • Protein/DNA gel and blotting equipment
  • Crystallization plate greaser
  • Ultra-cold freezers
  • Microscopes
  • and all kinds of other things....

Departmental Resources

We are lucky to have easy access to a variety of well-maintained, sophisticated equipment within the department.  These include:

  • Constant-flow pressure cell disrupter
  • MALDI-TOF MS and ESI-MS/MS mass spectrometry machines
  • Biacore surface plasmon resonance machine
  • Isothermal titration calorimeter
  • Circular dichroism machine
  • NMR facilities
  • Gel documentation imagers
  • Phosphoimager
  • Film developer
  • Various incubators
  • Various mid- and ultra-high-speed centrifuges
  • Cell culture facilities
  • Electroporator
  • 2-D gel rigs (and supporting instruments)
  • Large cold room
  • Sonicators

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This site was last updated 10/31/08