| Name |
cloning sites
(5', 3') |
Cloned Region |
ORF size (bp) |
Length (aas) |
Protein size
(Da) |
PCR success |
TA CLONE |
P1 clone |
P13 clone |
p22 clone |
p259 clone |
pichia (p270)
clone |
P1/P259
Expression |
p13
Expression |
p22
Expression |
Pichia
expression |
Notes |
| AF9 |
EcoRI, NotI |
full |
1704 |
568 |
63233 |
YES |
YES |
YES |
YES |
YES |
|
YES |
insoluble, poor
expression |
insoluble, poor
expression |
insoluble, poor
expression |
no |
P1 construct
shows strange restriction map |
| AMPH |
BamHI, NotI |
full |
2085 |
695 |
76127 |
no |
|
|
|
|
|
|
|
|
|
|
|
| ARA70 |
BamHI, NotI |
full |
1842 |
614 |
69629 |
YES |
YES |
YES |
YES |
YES |
|
YES |
insoluble, good
expression |
insoluble, good
expression |
insoluble, good
expression |
poor |
|
| BAF47 |
BamHI, NotI |
N-term |
876 |
292 |
33475 |
YES |
YES |
YES |
YES |
YES |
|
|
soluble, good
expression |
soluble, good
expression |
soluble, good
expression |
|
|
| BCAR3 |
EcoRI, NotI |
full |
2475 |
825 |
92442 |
YES |
YES |
YES |
YES |
YES |
|
YES |
insoluble, poor
expression |
insoluble, poor
expression |
no |
no |
|
| BCAS1 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| BCL10 |
BamHI, NotI |
full |
699 |
233 |
25901 |
YES |
YES |
YES |
YES |
YES |
|
|
YES |
insoluble |
YES, low
expression |
|
|
| BIN1 |
BamHI, NotI |
full |
1317 |
439 |
48214 |
YES |
YES |
YES |
YES |
YES |
|
|
YES |
YES |
YES |
|
|
| BNSP |
BamHI/NotI |
C-term |
798 |
266 |
31100 |
YES |
YES |
YES |
YES |
YES |
|
|
YES |
mass discrepancy |
mass discrepancy |
|
|
| BRCC3 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| BSP |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| CBFA2T1 |
SmaI/NotI |
full |
1701 |
567 |
63124 |
YES |
YES |
|
YES |
YES |
YES |
YES |
low expression,
soluble |
no |
no |
|
|
| CD19 |
BamHI/NotI |
C-term |
705 |
235 |
25364 |
YES |
YES |
YES |
YES |
YES |
|
|
low expression,
soluble |
low expression,
soluble |
low expression,
soluble |
|
|
| CDC10 |
BamHI/NotI |
C-term |
753 |
251 |
30799 |
YES |
YES |
YES |
YES |
YES |
|
|
YES (multiple
bands) |
no |
no |
|
|
| CTAG |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| DAB2 |
SmaI/NotI |
full |
2313 |
770 |
82338 |
YES |
YES |
YES |
YES |
YES |
|
|
YES, low
expression |
YES, low
expression |
YES, low
expression |
|
|
| DDIT3 |
BamHI, NotI |
full |
507 |
169 |
19136 |
YES |
YES |
YES |
YES |
YES |
|
|
YES |
insoluble |
insoluble |
|
|
| ELL |
BamHI, NotI |
full |
1920 |
640 |
72255 |
YES |
YES |
YES |
YES |
YES |
|
YES |
insoluble |
insoluble |
no expression |
no |
|
| EMS1 |
BamHI, NotI |
full |
1902 |
634 |
70862 |
YES |
YES |
YES |
YES |
YES |
|
|
degraded |
no |
no |
|
|
| EXT1 |
BamHI/NotI |
C-term |
2139 |
713 |
82434 |
YES |
YES |
YES |
YES |
YES |
|
|
no |
no |
no |
|
|
| EXT2 |
BamHI/NotI |
C-term |
2001 |
667 |
76258 |
YES |
YES |
YES |
YES |
YES |
|
YES |
insoluble |
insoluble |
|
|
|
| FBP |
EcoRI/NotI |
center |
339 |
113 |
13518 |
YES |
YES |
YES |
YES |
YES |
|
|
YES |
|
|
|
|
| FRAT1 |
BamHI/NotI |
C-term |
393 |
131 |
14007 |
YES |
YES |
YES |
YES |
YES |
|
|
YES (multiple
bands) |
insoluble |
insoluble |
|
|
| GAS41 |
BamHI/NotI |
C-term |
570 |
190 |
22387 |
YES |
YES |
YES |
YES |
YES |
|
|
sparingly soluble |
insoluble |
insoluble |
|
|
| HD21 |
BamHI/NotI |
full |
564 |
188 |
21519 |
YES |
YES |
YES |
YES |
YES |
|
|
YES |
YES, mass
discrepancy |
YES, mass
discrepancy |
|
|
| Hest |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| HMGIC |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| HR54 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| HRASLS |
BamHI/EcoRI |
full |
819 |
273 |
30117 |
YES |
YES |
YES |
YES |
YES |
|
YES |
|
|
|
no |
|
| HRP1 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| HSPB1 |
BamHI, NotI |
full |
615 |
205 |
22739 |
YES |
YES |
YES |
YES |
YES |
|
|
YES |
insoluble |
insoluble |
|
|
| IGF2AS |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| IGFBP9 |
BamHI/NotI |
C-term |
951 |
317 |
34690 |
YES |
YES |
YES |
YES |
YES |
|
|
sparingly soluble |
no |
insoluble |
|
|
| ING1 p24 |
EcoR1, BamHI |
full |
630 |
210 |
23699 |
N/A |
|
YES |
YES |
|
|
|
sparingly soluble |
insoluble |
|
|
possible mass
descrepancy |
| ING1 p33 |
EcoR1, BamHI |
full |
837 |
279 |
31810 |
N/A |
|
YES |
YES |
|
|
|
sparingly soluble |
insoluble |
|
|
|
| KUB3 |
EcoR1/NotI |
full |
738 |
246 |
28031 |
YES |
YES |
YES |
YES |
YES |
|
|
insoluble |
insoluble |
no |
|
|
| LMO2 |
BamHI, NotI |
full |
474 |
158 |
18319 |
YES |
YES |
YES |
YES |
YES |
|
YES |
insoluble |
insoluble |
no |
poor |
|
| LYL1 |
|
|
450 |
150 |
16531 |
no |
|
|
|
|
|
|
|
|
|
|
|
| MA2 |
BamHI, NotI |
full |
1092 |
364 |
41375 |
YES |
YES |
YES |
YES |
YES |
|
|
sparingly soluble |
sparingly soluble |
no |
|
|
| MTA1L1 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| MXI1 |
|
|
|
|
|
YES |
YES |
YES |
YES |
YES |
|
YES |
sparingly soluble |
insoluble, mass
problem |
no |
|
|
| NBS1 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| PAP |
BamHI, NotI |
full |
543 |
181 |
20495 |
YES |
YES |
YES |
YES |
YES |
|
|
YES |
insoluble |
insoluble |
|
|
| PDCD2 |
BamHI, XhoI |
full |
684 |
228 |
25485 |
no |
|
|
|
|
|
|
|
|
|
|
|
| PDGFRL |
BamHI, NotI |
full |
1125 |
375 |
41795 |
YES |
YES |
YES |
YES |
YES |
|
|
insoluble |
insoluble |
insoluble |
|
possible mass
descrepancy |
| PRCC |
SmaI, NotI |
full |
1473 |
491 |
52341 |
YES |
YES |
YES |
YES |
YES |
|
|
YES |
no |
YES |
|
|
| RASSF1 |
BamHI, NotI |
full |
810 |
270 |
31140 |
YES |
YES |
YES |
YES |
YES |
|
|
YES, low
expression |
no |
no |
|
|
| RBBP8 |
BamHI, NotI |
full |
2706 |
902 |
102526 |
YES |
YES |
YES |
YES |
YES |
|
YES |
insoluble |
insoluble |
insoluble |
no |
|
| RET43 |
SalI/NotI |
full |
|
|
|
YES |
YES |
|
YES |
YES |
YES |
|
insoluble |
no |
insoluble |
|
|
| RET51 |
SalI/NotI |
full |
|
|
|
YES |
YES |
|
YES |
YES |
YES |
|
insoluble |
no |
|
|
|
| RET9 |
SalI/NotI |
full |
|
|
|
YES |
YES |
YES |
YES |
YES |
|
|
sparingly soluble |
insoluble |
insoluble |
|
|
| SNCG |
BamHI, NotI |
full |
381 |
127 |
13298 |
YES |
YES |
YES |
YES |
YES |
|
|
YES |
YES |
no |
|
|
| SSX1 |
BamHI/NotI |
full |
564 |
188 |
21888 |
YES |
YES |
YES |
YES |
YES |
|
|
mass discrepancy |
mass discrepancy |
mass discrepancy |
|
|
| SSXT |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| ST5 |
EcoRI/NotI |
center |
2145 |
715 |
79600 |
YES |
YES |
YES |
YES |
YES |
|
YES |
no |
no |
no |
no |
|
| TFAP2B |
BamHI/NotI |
C-term |
645 |
215 |
2381 |
YES |
YES |
YES |
YES |
YES |
|
|
YES |
poor |
YES |
|
|
| TPD52 |
BamHI, NotI |
full |
552 |
184 |
19823 |
YES |
YES |
YES |
YES |
YES |
|
|
YES |
YES |
YES |
|
|
| DSCP1 |
SalI/NotI |
111-278 |
522 |
174 |
18083 |
YES |
YES |
YES |
YES |
YES |
|
YES |
|
|
|
|
|
| CA125
(HOST) |
SalI/NotI |
1-872 |
2616 |
872 |
96883 |
YES |
YES |
YES |
YES |
YES |
|
YES |
|
|
|
|
|
| PDRG |
BamHI/NotI |
full length |
399 |
133 |
15475 |
YES |
YES |
YES |
YES |
YES |
|
YES |
YES |
mass discrepancy |
mass discrepancy |
|
|
| BUB1 |
BamHI/NotI |
full length |
3150 |
1050 |
119354 |
YES |
no |
|
|
|
|
|
|
|
|
|
|
| bri3 |
BamHI/SalI |
1-60 |
180 |
60 |
6555 |
YES |
YES |
YES |
YES |
YES |
|
|
|
|
|
|
|
| TCP10L |
BamHI/NotI |
full length |
645 |
215 |
23818 |
YES |
YES |
YES |
YES |
YES |
|
YES |
YES |
mass discrepancy |
no |
|
|
| TOB/ERBB2 |
BamHI/NotI |
full length |
1035 |
345 |
38094 |
YES |
YES |
YES |
YES |
YES |
|
YES |
very weak |
mass discrepancy |
mass discrepancy |
|
|
| MT-SP1/matriptase |
BamHI/NotI |
83-855 |
2319 |
773 |
85710 |
YES |
YES |
YES |
YES |
YES |
|
YES |
no |
no |
no |
|
|
| KLK8/Neuropsin/Ovasin |
BamHI/NotI |
72-260 |
567 |
189 |
20375 |
YES |
no |
|
|
|
|
YES |
|
|
|
|
|
| PEG10 |
BamHI/NotI |
full length |
975 |
325 |
36906 |
YES |
YES |
YES |
YES |
YES |
|
YES |
very weak |
insoluble |
no |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|